跟着Plos Biology学作图:R语言ggplot2散点图和误差线

2022-04-08 13:48:39 浏览数 (1)

论文

Large variation in the association between seasonal antibiotic use and resistance across multiple bacterial species and antibiotic classes

数据代码链接

https://github.com/orgs/gradlab/repositories

今天的推文重复一下论文中的 Figure 3A

image.png

今天的推文主要有两个知识点

  • 一个是坐标轴文字同时存在斜体字和正常字应该怎么处理(上图的横坐标)
  • 二是坐标轴标题如果要换行应该怎么处理(上图的纵坐标)

准备作图数据的代码

代码语言:javascript复制
library(readr)
library(tidyverse)

res.modelA.params <- read_csv("use-resistance-seasonality/tables/resistance_modelA_values.csv")
filter_models_AIC_func = function(table, group_cols) {
  
  table.fil = table %>%
    group_by_at(vars(all_of(group_cols))) %>%
    mutate(rank = dense_rank(AIC)) %>%
    ungroup() %>%
    filter(rank == 1) %>%
    select(-AIC, -rank)
  
  return(table.fil)
}
res.modelA.params.fil <- filter_models_AIC_func(res.modelA.params, c("organism", "drug_name"))
f3a_data <- res.modelA.params.fil %>%
  filter(term == "amplitude") %>%
  mutate(org_drug = paste(organism, drug_code, sep = "/")) %>%
  select(org_drug, drug_class, period, term, estimate, ci.lower, ci.upper)

准备作图配色

代码语言:javascript复制
colors <- setNames(c("#220050", "#b30059","#0091a8","#359023", "#ffa500"),
                  c("Macrolides", "Nitrofurans", "Penicillins", "Quinolones", "Tetracyclines"))

定义横坐标文字标签

这里设置斜体使用expression()函数

代码语言:javascript复制
labs <- c("S. aureus/CIP" = expression(paste(italic("S. aureus"), " / CIP")),
         "S. aureus/ERY" = expression(paste(italic("S. aureus"), " / ERY")),
         "S. aureus/NIT" = expression(paste(italic("S. aureus"), " / NIT")),
         "S. aureus/OXA" = expression(paste(italic("S. aureus"), " / OXA")),
         "S. aureus/PEN" = expression(paste(italic("S. aureus"), " / PEN")),
         "S. aureus/TET" = expression(paste(italic("S. aureus"), " / TET")),
         "E. coli/AMC" = expression(paste(italic("E. coli"), " / AMC")),
         "E. coli/AMP" = expression(paste(italic("E. coli"), " / AMP")),
         "E. coli/CIP" = expression(paste(italic("E. coli"), " / CIP")),
         "E. coli/NIT" = expression(paste(italic("E. coli"), " / NIT")),
         "E. coli/TET" = expression(paste(italic("E. coli"), " / TET")),
         "K. pneumoniae/AMC" = expression(paste(italic("K. pneumoniae"), " / AMC")),
         "K. pneumoniae/CIP" = expression(paste(italic("K. pneumoniae"), " / CIP")),
         "K. pneumoniae/NIT" = expression(paste(italic("K. pneumoniae"), " / NIT")),
         "K. pneumoniae/TET" = expression(paste(italic("K. pneumoniae"), " / TET")))

画图代码

代码语言:javascript复制
f3a <- f3a_data %>%
  ggplot(aes(x = reorder(org_drug, estimate), y = estimate, color = drug_class))  
  geom_point(size = 2)  
  geom_errorbar(aes(ymin = ci.lower, ymax = ci.upper), width = 0.4)  
  geom_hline(yintercept = 0, color = "grey20", linetype = "dashed")  
  scale_y_continuous(breaks = c(0, 0.05, 0.1), limits = c(-0.02, 0.1))  
  scale_x_discrete(labels = labs)  
  scale_color_manual(values = colors, name = "Antibiotic Class")  
  ylab(expression(atop("Amplitude of seasonality", "(" ~ log["2"] ~ "(MIC) deviates)")))  
  theme_minimal()  
  theme(
    axis.title.x = element_blank(),
    axis.title.y = element_text(size = 10),
    axis.text.x = element_text(size = 10, angle = 45, hjust = 1, vjust = 1),
    axis.text.y = element_text(size = 10),
    legend.title = element_text(size = 9, face = "bold"),
    legend.text = element_text(size = 9),
  )

f3a

image.png

纵坐标轴标题换行的代码是ylab()这一行 expression(atop()),如果要换行就把不同的字符串用逗号分开,可以试下如下代码的效果

代码语言:javascript复制
ggplot() 
  geom_point(aes(x=1,y=1)) 
  ylab(expression(atop("ABCDE"," abcde" ~ "(" ~ ")")))

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