曾经写过97%阈值划分OTU的历史：
97%阈值的历史
说明了为什么97%的阈值可以对应到种水平。
那么在更高的分类水平呢，为啥95%可以对应到属水平，85%可以对应到纲水平，60-70%可以对应到门水平？
放几篇参考文献需要的时候看看。
1.Keswani J, Whitman WB (2001) Relationship of 16S rRNA sequence similarity to DNA hybridization in prokaryotes. Int J Syst Evol Microbiol 51: 667–678.
2.Sait M, Hugenholtz P, Janssen PH (2002) Cultivation of globally distributed soil bacteria from phylogenetic lineages previously only detected in cultivation-independent surveys. Environ Microbiol 4: 654–666.
3.Stackebrandt E, Goebel BM (1994) A place for DNA-DNA reassociation and 16S rRNA sequence-analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44: 846–849.
4.Everett KDE, Bush RM, Andersen AA (1999) Emended description of the order Chlamydiales, proposal of Parachlamydiaceae fam. nov. and Simkaniaceae fam. nov., each containing one monotypic genus, revised taxonomy of the family Chlamydiaceae, including a new genus and five new species, and standards for the identification of organisms. Int J Syst Bacteriol 49: 415–440.
5.Hugenholtz P, Goebel BM, Pace NR (1998) Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity. J Bacteriol 180: 4765–4774.
来源可看这篇:
Schloss PD, Handelsman J (2006) Toward a Census of Bacteria in Soil. PLoS Comput Biol 2(7): e92.https://doi.org/10.1371/journal.pcbi.0020092
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