生信论文的套路
- ONCOMINE从全景、亚型两个维度做表达差异分析；
- 临床标本从蛋白水平确认（或HPA数据库），很重要；
- Kaplan-Meier Plotter从临床意义的角度阐明其重要性；
- cBio-portal数据库做基因组学的分析（机制一）；
- STRING互作和GO/KEGG分析探讨可能的信号通路（机制二）；
- TISIDB/TIMER分析肿瘤免疫特征（机制三）。
引文的基本内容：介绍背景（一段或两段）、提出问题（一段），解决问题（一段或2段）。一般引文介绍3~5段。
- 疾病或基因的背景介绍；
- 前人研究的成果、现实情况，存在的问题；
- 阐明研究原因或意义。介绍研究的性质、范畴及其重要性，突出研究目的和待解决的科学问题；
- 研究方法，新发现和意义。
跟生信论文42中乳腺癌引文写作对比，该论文的引文写作更简单扼要，各部分描述比例均衡，而且过渡巧妙，流畅，值得借鉴和模仿。
生信论文43--肝癌--引文写作
第一段详细介绍肝癌的流行病学，预后现状和治疗情况，引出诊断和治疗的重要性。
Hepatocellular carcinoma (HCC) is the most common form of liver cancers [1], which has an annual incidence of at least 6 per 100,000 individuals and represents the fastest-rising cause of cancer-related death [2]. Due to the high rate of recurrence and metastasis, the five-year survival rate for advanced HCC is poor. However, existing targeted drugs show unsatisfactory efficacy, due to a combination of factors spanning an array of different clinical and biological behaviors, and the development of anti-HCC drug resistance [3]. The molecular mechanisms underlying tumor formation and progression are poorly understood, which further complicates the effective treatment of HCC [4]. In addition, the lack of markers that are specific for tumor type or disease stage represents a critical gap in the current understanding and treatment of HCC.
第二段通过Pre-mRNA剪切这一生化过程，并阐释其与肿瘤的关系，最后引出PRPF3基因的功能介绍。
Pre-mRNA splicing is a fundamental process that plays a considerable role in generating protein diversity. Pre-mRNA splicing is also the key to the pathology of numerous diseases, especially cancers [5]. The connection between cancer biology and splicing regulation is of primary importance to understand the mechanisms leading to disease and also to improve the development of therapeutic approaches [6]. Among the array of splicing factors, pre-mRNA processing factor 3 (PRPF3), a component of the U4/U6 di-snRNP, is required for U4/U6•U5 tri-snRNP formation and recruitment to active spliceosomes, which is essential for efficient pre-mRNA splicing [7, 8].
第三段通过PRPF3基因的深入描述，引出其在肝细胞发育和分化的作用，提出其在肝癌中作用的探究（重要性）。
It is also known that one gene pair, KCNE2-PRPF3 as the signature could robustly predict prognoses of gastric cancer patients treated with 5-FU-based chemotherapy [9]. As a member of the hepatic transcription factor network, Hepatocyte Nuclear Factor 4 Alpha (HNF4α) plays a pivotal role in liver development and hepatocellular differentiation. One study indicated that PRPF3 is an HNF4α regulated gene with induced expression in mouse and human HCC [10]. However, the biological function of PRPF3 in HCC remains to be determined.
第四段，解决问题，经典三句式。差异分析，组学分析，功能分析和免疫浸润分析。最后给出结论，简明扼要。
Here, we investigated PRPF3 expression and mutations in data from patients with HCC in The Cancer Genome Atlas (TCGA) and various public databases. Using multi-dimensional analysis, we evaluated genomic alterations and functional networks related to PRPF3 in HCC and explored its role in tumor immunity. Our results could potentially reveal new targets and strategies for HCC diagnosis and treatment.
