homer分析RNAseq数据

2020-04-01 15:48:27 浏览数 (1)

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Homer这个软件比较强大,主要做ChIP-Seq分析,除此之外,还可以做RNAseq以及microarray的分析

原文:http://homer.ucsd.edu/homer/motif/rnaMotifs.html

Analyzing Co-regulated Gene Lists for RNA motifs

用法:findMotifs.pl downregulated.genes.txt human-mRNA MotifOutput/ -rna -len 8

输入文件:3 mandatory arguments: A gene ID input file, the name of the promoter set (which is tied to an organism), and an output directory for all of the output files.

解释:输入文件downregulated.genes.txt为下调的gene list ,human-mRNA为promoter set,MotifOutput为输出文件夹

例子:

代码语言:javascript复制
for sample in `ls *txt |cut -d '.' -f1`;do findMotifs.pl ${sample}.txt human ${sample}/ -start -400 -end 100 -len 8,10 -p 16 ;done

结果:

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相应的promoter或者genome安装: 查看可以安装的文件:perl /path-to-homer/configureHomer.pl -list 安装或者卸载: "-install <package name>" or "-remove <package name>". 如: perl /path-to-homer/configureHomer.pl -install human 安装promoter则需要加“-p”,除此之外还有-o:organism -g genome perl /path-to-homer/configureHomer.pl -install human-p

参考:http://homer.ucsd.edu/homer/microarray/index.html

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